PROTEIN KINASE-A MODULATES CA2+-DEPENDENT AND PROTEIN KINASE-C-DEPENDENT AMYLASE RELEASE IN PERMEABILIZED RAT PANCREATIC ACINI

被引:25
作者
OSULLIVAN, AJ [1 ]
JAMIESON, JD [1 ]
机构
[1] YALE UNIV,SCH MED,DEPT CELL BIOL,C222 SHM,333 CEDAR ST,NEW HAVEN,CT 06510
关键词
D O I
10.1042/bj2870403
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of protein kinase A (PKA) in the release of amylase from permeabilized pancreatic acini was investigated. Addition of cyclic AMP (cAMP) to permeabilized acini resulted in a potentiation of Ca2+-dependent amylase release, shifting the Ca2+ dose/response curve leftwards. As with protein kinase C (PKC) activation, this is due to an increase in the time of active discharge. The effect of cAMP was shown to be blocked by two inhibitors of PKA, H89 and the PKI-(5-24)-peptide. At low concentration, cAMP synergizes from phorbol 12-myristate 13-acetate (PMA), while at optimal concentrations cAMP and PMA are additive. PKA and PKC appear to work via similar, but not identical mechanisms.
引用
收藏
页码:403 / 406
页数:4
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