REGENERATION OF TRANSGENIC, MICROSPORE-DERIVED, FERTILE BARLEY

被引：121

作者：

JAHNE, A ^{[1
]}

BECKER, D ^{[1
]}

BRETTSCHNEIDER, R ^{[1
]}

LORZ, H ^{[1
]}

机构：

[1] RESISTENZLABOR SAATEN UNION, D-33818 LEOPOLDSOHE, GERMANY

来源：

THEORETICAL AND APPLIED GENETICS | 1994年 / 89卷 / 04期

关键词：

BARLEY MICROSPORE CULTURE; PARTICLE BOMBARDMENT; FERTILE TRANSGENIC BARLEY PLANTS;

D O I：

10.1007/BF00225390

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We have developed a system for the biolistic transformation of barley using freshly-isolated microspores as the target tissue. Independent transformation events led, on average, to the recovery of one plant per 1x10(7) bombarded microspores. Putative transformants have been regenerated using phosphinothricin as a selective agent. R(0) plants have been transferred to soil approximately 2 months after bombardment. Integration of the marker genes bar and uidA has been confirmed by Southern analysis. The marker genes are inherited in all progeny plants confirming the expected homozygous nature of the R(0) plants.

引用

页码：525 / 533

页数：9

共 41 条

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