A SIMPLE AND EFFICIENT METHOD FOR SITE-DIRECTED MUTAGENESIS WITH DOUBLE-STRANDED PLASMID DNA

被引:10
作者
LAI, DS [1 ]
ZHU, XL [1 ]
PESTKA, S [1 ]
机构
[1] UMDNJ,ROBERT WOOD JOHNSON MED SCH,DEPT MOLEC GENET & MICROBIOL,PISCATAWAY,NJ 08854
关键词
D O I
10.1093/nar/21.17.3977
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high efficiency of mutation (58-97%) was obtained. If two synthetic oligonucleotide primers are used, two separate mutations can be simultaneously created in a single reaction tube.
引用
收藏
页码:3977 / 3980
页数:4
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