ELECTROSTATIC MANIPULATION OF DNA IN MICROFABRICATED STRUCTURES

被引:273
作者
WASHIZU, M [1 ]
KUROSAWA, O [1 ]
机构
[1] ADV CO,CHUO KU,TOKYO 103,JAPAN
关键词
DNA Dielectrophoresis - DNA Electrostatic Manipulation - Microfabricated Electrode System - Molecular Manipulation Techniques - Molecular Size Determination;
D O I
10.1109/28.62403
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Experimental observation of the electrostatic orientation and dielectrophoresis of DNA under a high-intensity field produced in a microfabricated electrode system is made, using the fluorescent method as a visualization technique. It is shown that the orientation takes place under a field strength of 106 V/m and the frequency at around 1 MHz. The stretch and shrink of an individual molecule is observed as the field is switched on and off. The molecular size (i.e., number of base pairs) is obtainable by measuring the stretched length of the molecule. A special field configuration is designed for the efficient molecular size determination using this principle. Molecular manipulation techniques, the alignment and positioning of the individual molecule, and the cutting of DNA at arbitrary locations with a UV beam are developed. © 1990 IEEE
引用
收藏
页码:1165 / 1172
页数:8
相关论文
共 24 条