BOVINE LEUKEMIA-VIRUS GENE-EXPRESSION INVIVO

被引：24

作者：

HAAS, L ^{[1
]}

DIVERS, T ^{[1
]}

CASEY, JW ^{[1
]}

机构：

[1] CORNELL UNIV, NEW YORK STATE COLL VET MED, DEPT MICROBIOL PARASITOL & IMMUNOL, ITHACA, NY 14853 USA

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 10期

关键词：

D O I：

10.1128/JVI.66.10.6223-6225.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as leukemia/lymphosarcoma develops.

引用

页码：6223 / 6225

页数：3

共 18 条

[1]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[2] INTEGRATION OF BOVINE LEUKEMIA-VIRUS AT ALL STAGES OF ENZOOTIC BOVINE LEUKOSIS [J].