PLASMID PKKH - AN IMPROVED VECTOR WITH HIGHER COPY NUMBER FOR EXPRESSION OF FOREIGN GENES IN ESCHERICHIA-COLI

被引：6

作者：

JING, GZ ^{[1
]}

HUANG, Z ^{[1
]}

LIU, ZG ^{[1
]}

ZOU, Q ^{[1
]}

机构：

[1] NATL VACCINE & SERUM INST,BEIJING 100024,PEOPLES R CHINA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 05期

关键词：

D O I：

10.1007/BF00129314

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An improved vector of 2889 bp was constructed by mutation of ropy number control system, into which foreign genes without or with the start codon ATG can be directly inserted for high-level expression in Escherichia coli. Deletion of the rop gene encoding a negative regulation protein ROP leads to increase the plasmid copy number, and finally makes the vector increase expression level more than 60% in comparison with initial one.

引用

页码：439 / 442

页数：4

共 8 条

[1] PROTEIN SECRETION CONTROLLED BY A SYNTHETIC GENE IN ESCHERICHIA-COLI [J].

BLANCHINROLAND, S ;

MASSON, JM .

PROTEIN ENGINEERING, 1989, 2 (06) :473-480

[2]

BOGOSIAN G, 1991, RECOMBINANT DNA TECH, P285

[3] PLASMID VECTORS FOR THE SELECTION OF PROMOTERS [J].

BROSIUS, J .

GENE, 1984, 27 (02) :151-160

[4] A NEW FAMILY OF SUGAR-INDUCIBLE EXPRESSION VECTORS FOR ESCHERICHIA-COLI [J].

CAGNON, C ;

VALVERDE, V ;

MASSON, JM .

PROTEIN ENGINEERING, 1991, 4 (07) :843-847

[5] REGULATION OF PLASMID COPY NUMBER BY COMPLEMENTARY RNAS [J].

CESARENI, G ;

BANNER, DW .

TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (08) :303-306

[6] HIGH-LEVEL EXPRESSION OF STAPHYLOCOCCAL NUCLEASE R-GENE IN ESCHERICHIA-COLI [J].

JING, GZ ;

LIU, LJ ;

JIANG, MY ;

QIANG, Z ;

HE, RQ .

JOURNAL OF BIOTECHNOLOGY, 1992, 22 (03) :271-282

[7] NOVEL METHOD FOR DETECTION OF BETA-LACTAMASES BY USING A CHROMOGENIC CEPHALOSPORIN SUBSTRATE [J].

OCALLAGHAN, CH ;

SHINGLER, AH ;

KIRBY, SM ;

MORRIS, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1972, 1 (04) :283-+

[8] A NOVEL PHOSPHATE-REGULATED EXPRESSION VECTOR IN ESCHERICHIA-COLI [J].

SU, TZ ;

SCHWEIZER, H ;

OXENDER, DL .

GENE, 1990, 90 (01) :129-133

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