THE ROLE OF THIOLS IN THE APPARENT ACTIVATION OF RAT-BRAIN NITRIC-OXIDE SYNTHASE (NOS)

被引：30

作者：

KOMORI, Y ^{[1
]}

HYUN, JJ ^{[1
]}

CHIANG, K ^{[1
]}

FUKUTO, JM ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,CTR HLTH SCI,SCH MED,DEPT PHARMACOL,LOS ANGELES,CA 90024

来源：

JOURNAL OF BIOCHEMISTRY | 1995年 / 117卷 / 04期

关键词：

DITHIOTHREITOL; NITRIC OXIDE SYNTHASE; PTERINS; TETRAHYDROBIOPTERIN; THIOL;

D O I：

10.1093/oxfordjournals.jbchem.a124797

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of thiols on the activation and/or stabilization of rat brain nitric oxide synthase (NOS) has been investigated. It was found that thiols are not necessary for stabilizing or protecting the protein during purification but are required during enzyme turnover for maximum activity. In the complete absence of thiols but with added tetrahydrobiopterin, the enzyme retained a low basal activity. Thiol addition to a thiol-deplete preparation of the enzyme resulted in a 4 to 7-fold increase in activity when measured after 15 min. High concentrations of dihydropteridine reductase also caused an apparent activation of NOS and was capable of replacing thiols. The data presented is consistent with a cofactor role for thiols. The possibility that they serve as reducing agents for the regeneration of tetrahydrobiopterin from dihydrobiopterins is discussed.

引用

页码：923 / 927

页数：5

共 22 条

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