LY-49A, A RECEPTOR FOR H-2D(D), HAS A FUNCTIONAL CARBOHYDRATE-RECOGNITION DOMAIN

被引：117

作者：

DANIELS, BF

NAKAMURA, MC

ROSEN, SD

YOKOYAMA, WM

SEAMAN, WE

机构：

[1] UNIV CALIF SAN FRANCISCO, DEPT IMMUNOL MICROBIOL, SAN FRANCISCO, CA 94143 USA

[2] UNIV CALIF SAN FRANCISCO, DEPT ANAT, SAN FRANCISCO, CA 94143 USA

[3] DEPT VET AFFAIRS HOSP, SAN FRANCISCO, CA 94121 USA

[4] MT SINAI MED CTR, HOWARD HUGHES MED INST, DEPT MED, NEW YORK, NY 10029 USA

[5] MT SINAI MED CTR, HOWARD HUGHES MED INST, DEPT MICROBIOL, NEW YORK, NY 10029 USA

[6] MT SINAI MED CTR, BROOKDALE CTR MOLEC BIOL, NEW YORK, NY 10029 USA

来源：

IMMUNITY | 1994年 / 1卷 / 09期

关键词：

D O I：

10.1016/S1074-7613(94)80020-0

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Ly-49A(+) murine natural killer (NK) cells cannot lyse target cells that express H-2D(d). We demonstrate a functional requirement for carbohydrate recognition by Ly-49A. Treatment of H-2D(d+) target cells with tunicamycin prevents their binding to Ly-49A(+) cells and renders them susceptible to lysis by Ly-49A(+) NK cells. Fucoidan, a sulfated polysaccharide, binds to Ly-49A in a calcium-dependent manner, and this binding is inhibited by monosaccharides, particularly sulfated hexoses. The inactivation of Ly-49A(+) NK cells by H-2D(d+) target cells is reversed in the presence of glucose 6-SO4. These results indicate that Ly-49A has a functional carbohydrate recognition domain and that target expression of carbohydrates alters their susceptibility to natural killing.

引用

页码：785 / 792

页数：8

共 46 条

[1]

AHRENS PB, 1987, J BIOL CHEM, V262, P7575

[2] NATURAL-KILLER CELLS DISCRIMINATE BETWEEN HIGH MANNOSE-TYPE AND COMPLEX-TYPE ASPARAGINE-LINKED OLIGOSACCHARIDES [J].