CHARACTERIZATION OF THE FACTOR E1BF FROM A RAT HEPATOMA THAT MODULATES RIBOSOMAL-RNA GENE-TRANSCRIPTION AND ITS RELATIONSHIP TO THE HUMAN KU AUTOANTIGEN

被引：22

作者：

HOFF, CM ^{[1
]}

JACOB, ST ^{[1
]}

机构：

[1] CHICAGO MED SCH, DEPT PHARMACOL & MOLEC BIOL, 3333 GREEN BAY RD, N CHICAGO, IL 60064 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified the DNA binding protein E1BF (Enhancer 1 Binding Factor) from the nuclei of Morris hepatoma 3924A. This protein, which consists of two polypeptides of 72 kDa and 85 kDa, binds to several DNA sequences involved in the regulation of rDNA transcription.UV-crosslinking studies show that interaction of the protein with DNA is primarily through the 72 kDa polypeptide. Purified E1BF can also modulate pol I-directed transcription in a cell-free systems derived from rat and mouse. E1BF is related to the human autoantigen Ku as demonstrated by immunological cross-reactivity with monoclonal antibodies directed against Ku p70 in Western blot and gel mobility shift assays. © 1993 Academic Press, Inc.

引用

页码：747 / 753

页数：7

共 31 条

[1]

BELL S, 1986, SCIENCE, V241, P1192

[2] GENOMIC ORGANIZATION OF RAT RDNA [J].

CHIKARAISHI, DM ;

BUCHANAN, L ;

DANNA, KJ ;

HARRINGTON, CA .

NUCLEIC ACIDS RESEARCH, 1983, 11 (18) :6437-6452

[3] THE TATA-BINDING PROTEIN AND ASSOCIATED FACTORS ARE INTEGRAL COMPONENTS OF THE RNA POLYMERASE-I TRANSCRIPTION FACTOR, SL1 [J].

COMAI, L ;

TANESE, N ;

TJIAN, R .

CELL, 1992, 68 (05) :965-976

[4] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[5]

DIXIT A, 1987, J BIOL CHEM, V262, P11616

[6]

FRANCOEUR AM, 1986, J IMMUNOL, V136, P1648

[7]

GARG LC, 1989, J BIOL CHEM, V264, P220

[8]

GHOSH AK, 1993, IN PRESS GENE

[9] IMMUNOLOCALIZATION OF KU-PROTEINS (P80 P70) - LOCALIZATION OF P70 TO NUCLEOLI AND PERIPHERY OF BOTH INTERPHASE NUCLEI AND METAPHASE CHROMOSOMES [J].

HIGASHIURA, M ;

SHIMIZU, Y ;

TANIMOTO, M ;

MORITA, T ;

YAGURA, T .

EXPERIMENTAL CELL RESEARCH, 1992, 201 (02) :444-451

[10] PURIFICATION AND CHARACTERIZATION OF A NOVEL FACTOR WHICH STIMULATES RAT RIBOSOMAL GENE-TRANSCRIPTION INVITRO BY INTERACTING WITH ENHANCER AND CORE PROMOTER ELEMENTS [J].

JI, Z ;

JACOB, ST .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (10) :5177-5186

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