ANTIGENIC VARIATION OF PARASITE-DERIVED ANTIGENS ON THE SURFACE OF BABESIA BOVIS-INFECTED ERYTHROCYTES

被引:53
作者
ALLRED, DR
CINQUE, RM
LANE, TJ
AHRENS, KP
机构
关键词
D O I
10.1128/IAI.62.1.91-98.1994
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The hemoparasite Babesia bovis antigenically alters the bovine erythrocyte membrane surface by expression of isolate-specific, parasite-derived polypeptides. To determine whether antigenic variation also occurred on the infected erythrocyte surface, a calf was infected once with parasitized erythrocytes carrying the C9.1 clonal line of B. bovis. In vitro cultures then were established periodically from the peripheral blood and analyzed with sequentially collected sera from the same animal. The surface reactivity of infected erythrocytes cultured from the infected animal varied over time, on the basis of reactivity in live cell immunofluorescence, surface immunoprecipitation, and panning assays. Subclones C8 and H10, established from day 41 cultures, were analyzed immunochemically. A loss of immunoreactivity was observed in antigens corresponding to the 113-and 128-kDa parasite-derived antigens of clone C9.1, demonstrating epitopic variation in these antigens; the immunochemical recognition of these antigens paralleled the results of live cell immunofluorescence and panning assays. Concomitant size polymorphism suggested polypeptide structural variation of these antigens as well. Calves infected by inoculation of infected blood or by injection of cloned parasites from in vitro cultures rapidly developed antibodies which cross-reacted among the clonal variant lines, suggesting the presence of common as well as unique epitopes. These results demonstrate that antigenic variation occurs on the surface of B. bovis-infected erythrocytes and that the parasite-derived antigens of 113 and 128 kDa compose at least a part of the antigens undergoing variation.
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页码:91 / 98
页数:8
相关论文
共 28 条
[1]   POLYPEPTIDES REACTIVE WITH ANTIBODIES ELUTED FROM THE SURFACE OF BABESIA-BOVIS-INFECTED ERYTHROCYTES [J].
AHRENS, KP ;
ALLRED, DR .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1992, 87 :21-26
[2]   STRUCTURAL ALTERATION OF THE MEMBRANE OF ERYTHROCYTES INFECTED WITH BABESIA-BOVIS [J].
AIKAWA, M ;
RABBEGE, J ;
UNI, S ;
RISTIC, M ;
MILLER, LH .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1985, 34 (01) :45-49
[3]   ISOLATE-SPECIFIC PARASITE ANTIGENS OF THE BABESIA-BOVIS-INFECTED ERYTHROCYTE SURFACE [J].
ALLRED, DR ;
HINES, SA ;
AHRENS, KP .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 60 (01) :121-132
[4]   A NONSUBJECTIVE ASSAY FOR ANTIGENIC MODIFICATIONS OF THE BABESIA-BOVIS-PARASITIZED ERYTHROCYTE SURFACE [J].
ALLRED, DR ;
AHRENS, KP .
JOURNAL OF PARASITOLOGY, 1993, 79 (02) :274-277
[5]  
ALLRED DR, UNPUBN
[6]   ERYTHROPHAGOCYTOSIS IN MALARIA - HOST DEFENSE OR MENACE TO THE MACROPHAGE [J].
ARESE, P ;
TURRINI, F ;
GINSBURG, H .
PARASITOLOGY TODAY, 1991, 7 (01) :25-28
[7]  
BIGGS BA, 1992, J IMMUNOL, V149, P2047
[8]   ASSESSMENT OF IMMUNE PHAGOCYTOSIS OF PLASMODIUM-FALCIPARUM INFECTED RED-BLOOD-CELLS BY HUMAN-MONOCYTES AND POLYMORPHONUCLEAR LEUKOCYTES - A METHOD FOR VISUALIZING INFECTED RED-BLOOD-CELLS INGESTED BY PHAGOCYTES [J].
CELADA, A ;
CRUCHAUD, A ;
PERRIN, LH .
JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 63 (02) :263-271
[9]   IN VITRO AGGLUTINATION OF BOVINE ERYTHROCYTES INFECTED WITH BABESIA ARGENTINA [J].
CURNOW, JA .
NATURE, 1968, 217 (5125) :267-&
[10]   STUDIES ON ANTIGENIC CHANGES AND STRAIN DIFFERENCES IN BABESIA-ARGENTINA INFECTIONS [J].
CURNOW, JA .
AUSTRALIAN VETERINARY JOURNAL, 1973, 49 (06) :279-283