QUATERNARY STRUCTURE OF VACCINIA VIRUS THYMIDINE KINASE

被引：17

作者：

BLACK, ME ^{[1
]}

HRUBY, DE ^{[1
]}

机构：

[1] OREGON STATE UNIV,CTR GENE RES & BIOTECHNOL,DEPT MICROBIOL,CORVALLIS,OR 97331

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)92005-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thymidine kinase enzymes isolated from a variety of sources are generally considered to have a native molecular weight of 80-90 kDa composed of two 40-45 kDa subunits. Although these parameters may accurately describe the atypical deoxypyrimidine kinases expressed by members of the Herpesviridae, the nucleotide sequences of thymidine kinase genes isolated from human, mouse, chicken and a variety of poxviruses (vaccinia virus, monkeypox virus, variola virus, fowlpox virus and capripoxvirus) predict molecular weights on the order of 20-25 kDa for the derived primary translation products. To resolve this apparent dilemma, velocity sedimentation centrifugation, gel filtration chromatography and protein cross-linking procedures were employed to provide experimental evidence that enzymatically-active vaccinia virus thymidine kinase is a homotetrameric complex of 20 kDa monomers with a native Mr of 80 kDa. © 1990.

引用

页码：1080 / 1086

页数：7

共 21 条

[1] FOWLPOX VIRUS THYMIDINE KINASE - NUCLEOTIDE-SEQUENCE AND RELATIONSHIPS TO OTHER THYMIDINE KINASES [J].

BOYLE, DB ;

COUPAR, BEH ;

GIBBS, AJ ;

SEIGMAN, LJ ;

BOTH, GW .

VIROLOGY, 1987, 156 (02) :355-365

[2] THE COMPLETE DNA-SEQUENCE OF VARICELLA-ZOSTER VIRUS [J].

DAVISON, AJ ;

SCOTT, JE .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1759-1816

[3] NUCLEOTIDE-SEQUENCE OF THE THYMIDINE KINASE GENE REGION OF MONKEYPOX AND VARIOLA VIRUSES [J].

ESPOSITO, JJ ;

KNIGHT, JC .

VIROLOGY, 1984, 135 (02) :561-567

[4] SEQUENCE, STRUCTURE AND PROMOTER CHARACTERIZATION OF THE HUMAN THYMIDINE KINASE GENE [J].

FLEMINGTON, E ;

BRADSHAW, HD ;

TRAINADORGE, V ;

SLAGEL, V ;

DEININGER, PL .

GENE, 1987, 52 (2-3) :267-277

[5] THE NUCLEOTIDE-SEQUENCE AROUND THE CAPRIPOXVIRUS THYMIDINE KINASE GENE REVEALS A GENE SHARED SPECIFICALLY WITH LEPORIPOXVIRUS [J].

GERSHON, PD ;

BLACK, DN .

JOURNAL OF GENERAL VIROLOGY, 1989, 70 :525-533

[6] MAPPING AND IDENTIFICATION OF THE VACCINIA VIRUS THYMIDINE KINASE GENE [J].

HRUBY, DE ;

BALL, LA .

JOURNAL OF VIROLOGY, 1982, 43 (02) :403-409

[7] INHIBITION OF VACCINIA VIRUS THYMIDINE KINASE BY THE DISTAL PRODUCTS OF ITS OWN METABOLIC PATHWAY [J].

HRUBY, DE .

VIRUS RESEARCH, 1985, 2 (02) :151-156

[8] CELL-FREE SYNTHESIS OF ENZYMATICALLY ACTIVE VACCINIA VIRUS THYMIDINE KINASE [J].

HRUBY, DE ;

BALL, LA .

VIROLOGY, 1981, 113 (02) :594-601

[9] PURIFICATION OF VACCINIA VIRUS-INDUCED THYMIDINE KINASE-ACTIVITY FROM [S-35]METHIONINE-LABELED CELLS [J].

KIT, S ;

JORGENSEN, GN ;

LIAV, A ;

ZASLAVSKY, V .

VIROLOGY, 1977, 77 (02) :661-676

[10] THE NUCLEOTIDE-SEQUENCE OF THE CHICKEN THYMIDINE KINASE GENE AND THE RELATIONSHIP OF ITS PREDICTED POLYPEPTIDE TO THAT OF THE VACCINIA VIRUS THYMIDINE KINASE [J].

KWOH, TJ ;

ENGLER, JA .

NUCLEIC ACIDS RESEARCH, 1984, 12 (09) :3959-3971

← 1 2 3 →