THE NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF PORCINE LIVER PROLINE-BETA-NAPHTHYLAMIDASE - EVIDENCE FOR THE IDENTITY WITH CARBOXYLESTERASE

被引：41

作者：

MATSUSHIMA, M ^{[1
]}

INOUE, H ^{[1
]}

ICHINOSE, M ^{[1
]}

TSUKADA, S ^{[1
]}

MIKI, K ^{[1
]}

KUROKAWA, K ^{[1
]}

TAKAHASHI, T ^{[1
]}

TAKAHASHI, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 1,BUNKYO KU,TOKYO 113,JAPAN

来源：

FEBS LETTERS | 1991年 / 293卷 / 1-2期

关键词：

PROLINE-BETA-NAPHTHYLAMIDASE; CARBOXYLESTERASE; CDNA CLONING;

D O I：

10.1016/0014-5793(91)81147-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone for porcine liver proline-beta-naphthylamidase was isolated and sequenced. The deduced amino acid sequence of 567 residues was highly homologous with those of carboxylesterases (EC 3.1.1.1) previously reported for other species. In addition, proline-beta-naphthylamidase purified from porcine liver was shown to have strong activity towards p-nitrophenylacetate, a representative substrate for carboxylesterases. These results suggest that proline-beta-naphthylamidase is identical with carboxylesterase.

引用

页码：37 / 41

页数：5

共 12 条

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