MICROANALYSIS OF BETA-CYCLODEXTRIN AND GLUCOSYL-BETA-CYCLODEXTRIN IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION

被引：20

作者：

KUBOTA, Y ^{[1
]}

FUKUDA, M ^{[1
]}

OHTSUJI, K ^{[1
]}

KOIZUMI, K ^{[1
]}

机构：

[1] MUKOGAWA WOMENS UNIV,FAC PHARMACEUT SCI,KOSHIEN KYUBAN CHO,NISHINOMIYA,HYOGO 663,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 201卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90179-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid chromatography with pulsed amperometric detection was applied to the determination of β-cyclodextrin (β-CD) and glucosyl (G)-β-CD in human plasma. They were well resolved from each other and from background components of plasma on a polymer-based reversed-phase column with 0.6% acetonitrile aqueous solution containing 1 mM sodium hydroxide as an eluent. The samples in the effluent were detected with a pulsed amperometric detector after postcolumn alkalization. The detection limits of β-CD and G-β-CD in plasma at a signal-to-noise ratio of 3 were 11 and 5 pmol, respectively. © 1992.

引用

页码：99 / 102

页数：4

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