DETERMINATION OF ACYCLOVIR BY ULTRAFILTRATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：29

作者：

NEBINGER, P ^{[1
]}

KOEL, M ^{[1
]}

机构：

[1] MED LAB DR ENZENAUER & DR WILHELM,W-4500 OSNABRUCK,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 619卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80128-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, sensitive and rapid high-performance liquid chromatographic (HPLC) procedure to determine total serum acyclovir concentrations is described. The assay involves a heat inactivation step at 56-degrees-C to prevent risk of infection, ultrafiltration as a pretreatment step prior to ion-pair reversed-phase liquid chromatography using guanosine as internal standard, and ultraviolet detection at 254 nm. This method has excellent recovery (97-100%), linearity (0.5-100 mg/1) and precision (1.2-8.0% coefficient of variation). The detection limit is 50 mug/l. The assay proved to be suitable for therapeutic drug monitoring of acyclovir.

引用

页码：342 / 344

页数：3

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