GTBP, A 160-KILODALTON PROTEIN ESSENTIAL FOR MISMATCH-BINDING ACTIVITY IN HUMAN-CELLS

被引：483

作者：

PALOMBO, F

GALLINARI, P

IACCARINO, I

LETTIERI, T

HUGHES, M

DARRIGO, A

TRUONG, O

HSUAN, JJ

JIRICNY, J

机构：

[1] IST RIC BIOL MOLEC P ANGELETTI, I-00040 POMEZIA, ITALY

[2] UCL, SCH MED, LUDWIG INST CANC RES, LONDON W1P 8BT, ENGLAND

来源：

SCIENCE | 1995年 / 268卷 / 5219期

关键词：

D O I：

10.1126/science.7604265

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA mismatch recognition and binding in human cells has been thought to be mediated by the hMSH2 protein. Here it is shown that the mismatch-binding factor consists of two distinct proteins, the 100-kilodalton hMSH2 and a 160-kilodalton polypeptide, GTBP (for G/T binding protein). Sequence analysis identified GTBP as a new member of the MutS homolog family. Both proteins are required for mismatch-specific binding, a result consistent with the finding that tumor-derived cell lines devoid of either protein are also devoid of mismatch-binding activity.

引用

页码：1912 / 1914

页数：3

共 30 条

[1] CLUES TO THE PATHOGENESIS OF FAMILIAL COLORECTAL-CANCER [J].