AMPLIFICATION AND EXPRESSION OF HETEROLOGOUS ONCOSTATIN-M IN CHINESE-HAMSTER OVARY CELLS

被引：29

作者：

MALIK, N

GRAVES, D

SHOYAB, M

PURCHIO, AF

机构：

[1] Bristol-Myers Squibb, Pharmaceutical Research Institute, Seattle

来源：

DNA AND CELL BIOLOGY | 1992年 / 11卷 / 06期

关键词：

D O I：

10.1089/dna.1992.11.453

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A stable Chinese hamster ovary (CHO) cell line producing high levels of human oncostatin M (OM) was generated by transfecting a heterologous gene coding for the protein. This novel construct was comprised of the gene for the transforming growth factor-beta(1) (TGF-beta(1)) signal peptide fused to the gene for mature human OM. Amplification with methotrexate produced milligram quantities of this recombinant OM, which was processed correctly, glycosylated, and found to have biological functions similar to those of natural OM.

引用

页码：453 / 459

页数：7

共 26 条

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