A COLLAGEN RECEPTOR ON STAPHYLOCOCCUS-AUREUS STRAINS ISOLATED FROM PATIENTS WITH SEPTIC ARTHRITIS MEDIATES ADHESION TO CARTILAGE

被引：157

作者：

SWITALSKI, LM

PATTI, JM

BUTCHER, W

GRISTINA, AG

SPEZIALE, P

HOOK, M

机构：

[1] UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294

[2] UNIV ALABAMA,DEPT BIOCHEM,BIRMINGHAM,AL 35294

[3] MUSCULOSKELETAL SCI RES INST,HERNDON,VA 22071

[4] UNIV PAVIA,DEPT BIOCHEM,I-27100 PAVIA,ITALY

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 7卷 / 01期

关键词：

D O I：

10.1111/j.1365-2958.1993.tb01101.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Staphylococcus aureus strains isolated from patients with septic arthritis or osteomyelitis possess a collagen receptor present in two forms, which contains either two or three copies of a 187-amino-acid repeat motif. Collagen receptor-positive strains adhered to both collagen substrata and cartilage in a time-dependent process. Collagen receptor-specific antibodies blocked bacterial adherence, as did preincubation of the substrate with a recombinant form of the receptor protein. Furthermore, polystyrene beads coated with the collagen receptor bound collagen and attached to cartilage. Taken together, these results suggest that the collagen receptor is both necessary and sufficient to mediate bacterial adherence to cartilage in a process that constitutes an important part of the pathogenic mechanism in septic arthritis.

引用

页码：99 / 107

页数：9

共 28 条

[1]

BAUR S, 1972, J IMMUNOL, V108, P748

[2] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].