CLONING AND FUNCTIONAL EXPRESSION OF HUMAN ENDOTHELIN-CONVERTING ENZYME CDNA

被引：119

作者：

SHIMADA, K ^{[1
]}

MATSUSHITA, Y ^{[1
]}

WAKABAYASHI, K ^{[1
]}

TAKAHASHI, M ^{[1
]}

MATSUBARA, A ^{[1
]}

IIJIMA, Y ^{[1
]}

TANZAWA, K ^{[1
]}

机构：

[1] SANKYO CO LTD,PHARMACOL & MOLEC BIOL RES LABS,SHINJUKU KU,TOKYO 140,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 207卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1258

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endothelin (ET) is a 21-residue potent vasoconstrictive peptide produced by vascular endothelial cells and formed from its precursor, big endothelin (big ET), by endothelin-converting enzyme (ECE). This paper describes the cloning and functional expression of a cDNA encoding a human ECE from human umbilical vein endothelial cells (HUVEC). Human ECE consists of 758 amino acid residues and has high homology to rat and bovine ECE. Immunoblot analysis using a monoclonal antibody risen against rat lung ECE showed the presence of immunoreactive protein in membrane fraction prepared from both HUVEC and COS-1 cells transfected with human ECE cDNA. Both COS-1 cells expressing human ECE and its membrane fraction converted big ET-1 most efficiently among big ETs. (C) 1995 Academic Press, Inc.

引用

页码：807 / 812

页数：6

共 11 条

[1] IKURA T, 1994, BIOCH BIOPHIS RES CO, V20, P1417
[2] INOUE A, 1988, P NATL ACAD SCI USA, V86, P2863
[3] CONVERSION OF BIG ENDOTHELIN-1 TO 21-RESIDUE ENDOTHELIN-1 IS ESSENTIAL FOR EXPRESSION OF FULL VASOCONSTRICTOR ACTIVITY - STRUCTURE ACTIVITY RELATIONSHIPS OF BIG ENDOTHELIN-1
KIMURA, S
KASUYA, Y
SAWAMURA, T
SHINMI, O
SUGITA, Y
YANAGISAWA, M
GOTO, K
MASAKI, T
[J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1989, 13 : S5 - S7
[4] AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS
KOZAK, M
[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (20) : 8125 - 8148
[5] SANGER F, 1977, P NATL ACAD SCI USA, V83, P5463
[6] SHIMADA K, 1994, J BIOL CHEM, V269, P18275
[7] A SANDWICH-TYPE ENZYME-IMMUNOASSAY TO DETECT IMMUNOREACTIVE BIG-ENDOTHELIN-1 IN PLASMA
SUZUKI, N
MATSUMOTO, H
KITADA, C
KIMURA, S
MIYAUCHI, T
FUJINO, M
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 127 (02) : 165 - 170
[8] TAKAHASHI M, 1993, J BIOL CHEM, V26, P21394
[9] SR-ALPHA PROMOTER - AN EFFICIENT AND VERSATILE MAMMALIAN CDNA EXPRESSION SYSTEM COMPOSED OF THE SIMIAN VIRUS-40 EARLY PROMOTER AND THE R-U5 SEGMENT OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 LONG TERMINAL REPEAT
TAKEBE, Y
SEIKI, M
FUJISAWA, JI
HOY, P
YOKOTA, K
ARAI, KI
YOSHIDA, M
ARAI, N
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (01) : 466 - 472
[10] ECE-1 - A MEMBRANE-BOUND METALLOPROTEASE THAT CATALYZES THE PROTEOLYTIC ACTIVATION OF BIG ENDOTHELIN-1
XU, D
EMOTO, N
GIAID, A
SLAUGHTER, C
KAW, S
DEWIT, D
YANAGISAWA, M
[J]. CELL, 1994, 78 (03) : 473 - 485

← 1 2 →