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STUDIES ON NEUROSTEROIDS .1. RETENTION BEHAVIOR OF DERIVATIZED 17-OXOSTEROIDS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10
作者：
SHIMADA, K
SATOH, Y
NISHIMURA, S
机构：
[1] Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa 920
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 04期
关键词：
D O I：
10.1080/10826079508009267
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The retention behavior of five 17-oxosteroids (dehydroepiandrosterone, epiandrosterone, androsterone, 5 beta-androsterone, and 5 beta-androstane-3 beta ol-17-one) with 5-dimethylamino-1-naphthalenesulfonic hydrazide, 4-(N, N-dimethylaminosulfonyl)- 7-hydrazino-2, 1, 3-benzoxadiazole or p-nitrophenylhydrazine are examined using reversed-phase high-performance liquid chromatography. Inclusion chromatography using cyclodextrin as a mobile phase additive is also used for this purpose and found effective in separating the isomeric derivatized 17-oxosteroids.
引用
收藏
页码：713 / 723
页数：11
相关论文
共 6 条
[1]   NEUROSTEROID METABOLISM - 7-ALPHA-HYDROXYLATION OF DEHYDROEPIANDROSTERONE AND PREGNENOLONE BY RAT-BRAIN MICROSOMES [J].
AKWA, Y ;
MORFIN, RF ;
ROBEL, P ;
BAULIEU, EE .
BIOCHEMICAL JOURNAL, 1992, 288 :959-964
[2]   DETERMINATION OF 17-OXOSTEROIDS IN SERUM AND URINE BY FLUORESCENCE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING DANSYL HYDRAZINE AS A PRE-LABELING REAGENT [J].
KAWASAKI, T ;
MAEDA, M ;
TSUJI, A .
JOURNAL OF CHROMATOGRAPHY, 1981, 226 (01) :1-12
[3]   STEROIDS AND THEIR CONJUGATES IN THE MAMMALIAN BRAIN [J].
MATHUR, C ;
PRASAD, VVK ;
RAJU, VS ;
WELCH, M ;
LIEBERMAN, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (01) :85-88
[4]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF SENSITIVE FLUORESCENT DERIVATIVES OF BILE-ACIDS WITH CYCLODEXTRIN-CONTAINING MOBILE-PHASE [J].
SHIMADA, K ;
MITAMURA, K ;
ISHITOYA, S ;
HIRAKATA, K .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (18) :3965-3976
[5]   RETENTION BEHAVIOR OF CARDIAC STEROIDS USING CYCLODEXTRIN IN THE MOBILE PHASE IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
SHIMADA, K ;
OE, T ;
HIROSE, Y ;
KOMINE, Y .
JOURNAL OF CHROMATOGRAPHY, 1989, 478 (02) :339-347
[6]   DETERMINATION OF MEDROXYPROGESTERONE ACETATE IN SERUM BY HPLC WITH PEROXYOXALATE CHEMILUMINESCENCE DETECTION USING A FLUOROGENIC REAGENT, 4-(N,N-DIMETHYLAMINOSULPHONYL)-7-HYDRAZINO-2,1,3-BENZOXADIAZOLE [J].
UZU, S ;
IMAI, K ;
NAKASHIMA, K ;
AKIYAMA, S .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1992, 10 (10-12) :979-984
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