H2O2 DETERMINATION IN RAT LENS - CHEMILUMINESCENT VERSUS RADIOISOTOPIC METHODS

被引：10

作者：

DEVAMANOHARAN, PS ^{[1
]}

VARMA, SD ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT OPHTHALMOL,BALTIMORE,MD 21201

来源：

OPHTHALMIC RESEARCH | 1995年 / 27卷

关键词：

HYDROGEN PEROXIDE; CHEMILUMINESCENCE; RADIOISOTOPIC METHOD; DICHLOROPHENOL-INDOPHENOL; RAT LENS;

D O I：

10.1159/000267837

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

Hydrogen peroxide levels have been determined in rat lenses by using two methods, a chemiluminescent and a radioisotopic method. The average content was found to be 155+/-20 and 127 +/-18 nmol/g wet weight of the tissue, respectively, by the two methods. The reaction of H2O2 with dichlorophenol-indophenol in the presence of peroxidase was also studied. However, this was found to be less suitable. The results of the chemiluminescent determinations are similar to the results with the radioisotopic methods demonstrating the feasibility of determining H2O2 by chemiluminescence measurement as well.

引用

页码：39 / 43

页数：5

共 14 条

[1]

REGULATION OF HYDROGEN-PEROXIDE IN EYE HUMORS - EFFECT OF 3-AMINO-1H-1,2,4-TRIAZOLE ON CATALASE AND GLUTATHIONE PEROXIDASE OF RABBIT EYE [J].