A CONSERVED SEQUENCE MOTIF AT THE 5'-TERMINUS OF THE SOUTHAMPTON VIRUS GENOME IS CHARACTERISTIC OF THE CALICIVIRIDAE

被引：34

作者：

LAMBDEN, PR

LIU, BL

CLARKE, IN

机构：

[1] Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton

来源：

VIRUS GENES | 1995年 / 10卷 / 02期

基金：

英国惠康基金;

关键词：

CALICIVIRUS; SOUTHAMPTON VIRUS; GENOME ORGANIZATION; 5' TERMINAL MOTIF;

D O I：

10.1007/BF01702595

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have determined the 5' terminal cDNA sequence for the genome of Southampton virus, a recently characterized, human, small round-structured virus (SRSV). Genomic RNA was extracted directly from a stool sample and amplified by RT-PCR by homopolymer tailing of the 3' terminus of the cDNA. The additional sequence increases the overall length of the Southampton virus genome by 12 nucleotides, resulting in a significant change to the genome organization by extending the first large open reading frame (ORF) by 51 amino acids, The 5' terminal bases pGpT and the presence of conserved genome and putative subgenomic RNA terminal motifs are now prominent features shared between the human SRSV Southampton virus and the animal caliciviruses rabbit hemorrhagic disease virus and feline calicivirus.

引用

页码：149 / 152

页数：4

共 17 条

[1] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].