DETERMINATION OF HIGH-ENERGY PHOSPHATE-COMPOUNDS IN FISH MUSCLE - P-31-NMR SPECTROSCOPY AND ENZYMATIC METHODS

被引：18

作者：

VANDENTHILLART, G ^{[1
]}

VANWAARDE, A ^{[1
]}

MULLER, HJ ^{[1
]}

ERKELENS, C ^{[1
]}

LUGTENBURG, J ^{[1
]}

机构：

[1] LEIDEN STATE UNIV, GORLAEUS LABS, DEPT ORGAN CHEM, 2300 RA LEIDEN, NETHERLANDS

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 95卷 / 04期

关键词：

D O I：

10.1016/0305-0491(90)90318-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. High-energy phosphate compounds were measured in the lateral red and epaxial white muscles of goldfish by: (1) in vivo 31P-NMR spectroscopy; (2) in vitro 31P-NMR spectroscopy of excised tissue; (3) in vitro 31P-NMR spectroscopy of perchloric acid extracts and (4) enzymatic analysis of perchloric acid extracts. 2. 2. A rapid breakdown of phosphocreatine and accumulation of inorganic phosphate was observed during handling, tissue excision and extraction. 3. 3. The lability of phosphocreatine may explain the well-known differences between in vivo NMR spectra and spectra of perchloric acid extracts. 4. 4. A modified procedure in described for extraction of frozen tissue samples, resulting in 100% yield of phosphocreatine, ATP and inorganic phosphate, as judged by the recovery of internal standards. © 1990.

引用

页码：789 / 795

页数：7

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