TESTING ANOPHELES-ALBIMANUS FOR GENETIC-LINKAGE OF INSECTICIDE RESISTANCE GENES BY COMBINING INSECTICIDE BIOASSAY AND BIOCHEMICAL METHODS

被引：5

作者：

LINES, JD

FFRENCHCONSTANT, RH

KASIM, SH

机构：

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1990年 / 4卷 / 04期

关键词：

acetyl‐cholinesterase; Anopheles albimanus; bioassay; biochemical assay; dieldrin; insecticide resistance; propoxur;

D O I：

10.1111/j.1365-2915.1990.tb00464.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Abstract. A microtitre‐plate assay which distinguishes propoxur‐resistant from susceptible Anopheles albimanus Weidemann was used to test for linkage between the genes for propoxur‐ and dieldrin‐resistance. The adult progeny of a backcross between a doubly‐resistant colony and a fully susceptible colony were exposed in conventional test kits to the standard discriminating dose of dieldrin, and kept in the insectary overnight. Both live and dead insects were then assayed individually for propoxur‐resistance. The results showed that heterozygotes for propoxur‐resistance could be reliably distinguished from susceptibles whether or not they had been killed up to 24 h previously by dieldrin treatment. In this way all the backcross progeny could be scored at both resistance loci, and all four genotypic classes identified. Resistant and susceptible alleles at the two loci were inherited independently, demonstrating the absence of linkage. The usual method of testing for linkage between resistance genes is inefficient and open to bias, because insects have to be exposed to each insecticide in turn, and only half of them can be scored at both loci. The method shown here avoids these drawbacks. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：445 / 450

页数：6

共 15 条

[1]

Bonning B., (1989)

[2]

Brown T.M., Brogdon W.G., Improved detection of insecticide resistance through conventional and molecular techniques, Annual Review of Entomology, 32, pp. 145-162, (1987)

[3]

Curtis C.F., Theoretical models of the use of insecticide mixtures for management of resistance, Bulletin of Entomological Research, 75, pp. 259-265, (1985)

[4]

Davidson G., Sawyer B., Carbamate and Organophosphate resistance in Anopheles albimanus, Transactions of the Royal Society for Tropical Medicine and Hygiene, 69, (1975)

[5]

Ffrench-Constant R.H, Bonning B., Rapid mierotitre plate test distinguishes insecticide resistant acetylcholinesterase genotypes in the mosquitoes Anopheles albimanus, An.nigerrimus and Culex pipiens, Medical and Veterinary Entomology, 3, pp. 9-16, (1989)

[6]

Ffrench-Constant R.H., Devonshire A.L., A multiple homogenizer for rapid sample preparation in immunoassays and electrophoresis, Biochemical Genetics, 25, pp. 493-499, (1987)

[7]

Georghiou G., The evolution of resistance to pesticides, Annual Review of Ecology and Systematics, 3, pp. 133-168, (1972)

[8]

Haridi A.M., Linkage studies on DDT and dieldrin resistance in species A and B of the Anopheles gambiae complex, Bulletin of the World Health Organization, 50, pp. 441-448, (1974)

[9]

Hemingway J., Smith C, Jayawardena K.G.I., Herath P.R.J., Field and laboratory detection of the altered acetylcholinesterase resistance genes which confer organophosphate and carbamate resistance in mosquitoes (Diptera: Culicidac), Bulletin of Entomological Research., 76, pp. 559-565, (1986)

[10]

Lines J.D., Curtis C.F., Genetic linkage between malathion and dieldrin resistance in Anopheles arabiensis, Canadian Journal of Genetics and Cytology, 26, pp. 646-650, (1984)

← 1 2 →