BLOCKAGE OF AMV REVERSE-TRANSCRIPTASE BY ANTISENSE OLIGODEOXYNUCLEOTIDES

被引：7

作者：

LOREAU, N

BOIZIAU, C

VERSPIEREN, P

SHIRE, D

TOULME, JJ

机构：

[1] MUSEUM NATL HIST NAT,BIOPHYS LAB,CNRS,UA 481,INSERM,U201,F-75231 PARIS 05,FRANCE

[2] SANOFI ELF BIORECH,F-31328 LABEGE,FRANCE

来源：

FEBS LETTERS | 1990年 / 274卷 / 1-2期

关键词：

Antisense oligonucleotide; Intercalating agent; Rabbit globin; Reverse transcriptase; Trypanosoma brucei;

D O I：

10.1016/0014-5793(90)81327-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthetic oligodeoxynucleotides, either unmodified or linked to an intercalating agent, have been used to prevent cDNA elongation by the AMV reverse transcriptase. Oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA part bound to the antisense oligonucleotide by the RNase-H activity associated with the retroviral polymerase. © 1990.

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页码：53 / 56

页数：4

相关论文

共 21 条

[1] OLIGODEOXYNUCLEOTIDES COVALENTLY LINKED TO INTERCALATING DYES AS BASE SEQUENCE-SPECIFIC LIGANDS - INFLUENCE OF DYE ATTACHMENT SITE [J].

ASSELINE, U ;

TOULME, F ;

THUONG, NT ;

DELARUE, M ;

MONTENAYGARESTIER, T ;

HELENE, C .

EMBO JOURNAL, 1984, 3 (04) :795-800

[2] OLIGOTHYMIDYLATES SUBSTITUTED IN 3' POSITION BY AN ACRIDINE DERIVATIVE [J].

ASSELINE, U ;

THUONG, NT ;

HELENE, C .

NUCLEOSIDES & NUCLEOTIDES, 1986, 5 (01) :45-63

[3] ALPHA-ANOMERIC DNA - BETA-RNA HYBRIDS AS NEW SYNTHETIC INHIBITORS OF ESCHERICHIA-COLI RNASE-H, DROSOPHILA EMBRYO RNASE-H AND M-MLV REVERSE-TRANSCRIPTASE [J].

BLOCH, E ;

LAVIGNON, M ;

BERTRAND, JR ;

POGNAN, F ;

MORVAN, F ;

MALVY, C ;

RAYNER, B ;

IMBACH, JL ;

PAOLETTI, C .

GENE, 1988, 72 (1-2) :349-360

[4] DISCONTINUOUS TRANSCRIPTION AND ANTIGENIC VARIATION IN TRYPANOSOMES [J].

BORST, P .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :701-732

[5] ENZYMATIC AMPLIFICATION OF TRANSLATION INHIBITION OF RABBIT BETA-GLOBIN MESSENGER-RNA MEDIATED BY ANTI-MESSENGER OLIGODEOXYNUCLEOTIDES COVALENTLY LINKED TO INTERCALATING AGENTS [J].

CAZENAVE, C ;

LOREAU, N ;

THUONG, NT ;

TOULME, JJ ;

HELENE, C .

NUCLEIC ACIDS RESEARCH, 1987, 15 (12) :4717-4736

[6] INHIBITION OF DNA-SYNTHESIS BY CROSS-LINKING THE TEMPLATE TO PLATINUM-THIOL DERIVATIVES OF COMPLEMENTARY OLIGODEOXYNUCLEOTIDES [J].

CHU, BCF ;

ORGEL, LE .

NUCLEIC ACIDS RESEARCH, 1989, 17 (12) :4783-4798

[7] THE COMMON 5' TERMINAL SEQUENCE ON TRYPANOSOME MESSENGER-RNAS - A TARGET FOR ANTI-MESSENGER OLIGODEOXYNUCLEOTIDES [J].

CORNELISSEN, AWCA ;

VERSPIEREN, MP ;

TOULME, JJ ;

SWINKELS, BW ;

BORST, P .

NUCLEIC ACIDS RESEARCH, 1986, 14 (14) :5605-5614

[8] ALPHA-DNA .9. PARALLEL ANNEALING OF ALPHA-ANOMERIC OLIGODEOXYRIBONUCLEOTIDES TO NATURAL MESSENGER-RNA IS REQUIRED FOR INTERFERENCE IN RNASE-H MEDIATED HYDROLYSIS AND REVERSE TRANSCRIPTION [J].

GAGNOR, C ;

RAYNER, B ;

LEONETTI, JP ;

IMBACH, JL ;

LEBLEU, B .

NUCLEIC ACIDS RESEARCH, 1989, 17 (13) :5107-5114

[9] SPECIFIC REGULATION OF GENE-EXPRESSION BY ANTISENSE, SENSE AND ANTIGENE NUCLEIC-ACIDS [J].

HELENE, C ;

TOULME, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (02) :99-125

[10] INVIVO LABELING OF INTERMEDIATES IN THE DISCONTINUOUS SYNTHESIS OF MESSENGER-RNAS IN TRYPANOSOMA-BRUCEI [J].

LAIRD, PW ;

ZOMERDIJK, JCBM ;

DEKORTE, D ;

BORST, P .

EMBO JOURNAL, 1987, 6 (04) :1055-1062