PURIFICATION OF A NOVEL FACTOR WHICH BINDS TO THE MOUSE ALPHA-2 (I) COLLAGEN PROMOTER

被引：4

作者：

HATAMOCHI, A

DECROMBRUGGHE, B

KRIEG, T

机构：

[1] UNIV MUNICH, DERMATOL KLIN & POLIKLIN, W-8000 MUNICH 2, GERMANY

[2] UNIV TEXAS, MD ANDERSON CANC CTR, DEPT MOLEC GENET, HOUSTON, TX 77030 USA

来源：

FEBS LETTERS | 1993年 / 327卷 / 03期

关键词：

DNA BINDING FACTOR; MOUSE-ALPHA-2 (I) COLLAGEN PROMOTER; NIH-3T3; FIBROBLAST;

D O I：

10.1016/0014-5793(93)81014-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified and purified a DNA binding protein which specifically binds to a segment of the mouse alpha2 (I) collagen promoter between -420 and -399 bp upstream of the start of transcription. Purification included heparin-agarose and sequence-specific DNA-affinity chromatography, followed by SDS-PAGE and renaturation of the DNA binding activity after elution from SDS-polyacrylamide gel. The DNA binding activity resides in two species of 42 kDa and 40 kDa, respectively. The levels of DNA binding activity of this factor, which has been tentatively designated as ColF1, are considerably higher in nuclear extracts of NIH-3T3 fibroblasts than in nuclear extracts from epidermal cells, lymphoid cells and transformed NIH-3T3 fibroblasts.

引用

页码：325 / 331

页数：7

共 25 条

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[2]

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