THERMOOPTIC SPECTROSCOPY - NEW AND SENSITIVE SCHEMES FOR DETECTION IN CAPILLARY SEPARATION TECHNIQUES

被引:9
作者
SAZ, JM [1 ]
DIEZMASA, JC [1 ]
机构
[1] CSIC,INST ORGAN CHEM,E-28006 MADRID,SPAIN
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 03期
关键词
D O I
10.1080/10826079408013156
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Detection is a major problem in the application of Open-Tubular Capillary Liquid Chromatography (OTCLC) and Capillary Electrophoresis (CE) for analyzing very dilute samples, for instance in trace analysis. Thermo-optical Spectroscopy is a new branch of Spectroscopy which allows the detection of samples with low light absorbances (10(-8) - 10(-9) AU) using a few picoliters sample cell. A few attomoles of analyte can be detected in OTCLC or in CE with these techniques. These results are similar to those achieved by Laser-Induced Fluorescence detection in open-tubular capillary tubings. However, many organic substances present a high absorption coefficient and only a few of them provide good quantum yields. The principles of Thermo-optical Spectroscopy are presented in this work. The main thermo-optical methods and their application in OTCLC and CE are also reviewed.
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页码:499 / 520
页数:22
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