CHARACTERIZATION OF 3 PUTATIVE SERPULINA-HYODYSENTERIAE HEMOLYSINS

被引：54

作者：

TERHUURNE, AAHM

MUIR, S

VANHOUTEN, M

VANDERZEIJST, BAM

GAASTRA, W

KUSTERS, JG

机构：

[1] Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Utrecht

来源：

MICROBIAL PATHOGENESIS | 1994年 / 16卷 / 04期

关键词：

SERPULINA HYODYSENTERIAE; HEMOLYSIN; HEMOLYTIC ACTIVITY; CLONING; SWINE DYSENTERY; CLP PROTEIN;

D O I：

10.1006/mpat.1994.1028

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Serpulina hyodysenteriae hemolysin is thought to be an important virulence factor in swine dysentery. One gene, tlyA, previously called tly, encoding a hemolysin in S. hyodysenteriae strain B204 has been characterized (Muir et al. infect Immun 1992; 60: 529-35). Two other genes of S. hyodysenteria strain B204, designated tlyB and tlyC, encoding hemolytic proteins in Escherichia coli strain DH5 alpha were cloned and sequenced. The tlyB and tlyC genes, when expressed in E. coli, encode heat-labile, protease-sensitive proteins which exhibit both hemolytic and cytotoxic activity in vitro. The calculated molecular weights of the tlyB and tlyC gene products are 93.3 kDa and 30.8 kDa, respectively. The tlyB gene product has sequence homology with the Clp proteins, whereas for the tlyC-encoded protein no homology with other protein sequences was observed. Southern hybridization showed that the tlyB and tlyC genes are present in a single copy on the chromosome of S. hyodysenteriae.

引用

页码：269 / 282

页数：14

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