ISOLATION OF ANTIFUNGAL AND LARVICIDAL CONSTITUENTS OF DIPLOLOPHIUM-BUCHANANI BY CENTRIFUGAL PARTITION CHROMATOGRAPHY

Three phenylpropanoids, myristicin [1], elemicin [2], and trans-isoelemicin [3], together with two furanocoumarins, oxypeucedanin [4] and oxypeucedanin hydrate [5], have been isolated from the leaves of Diplolophium buchanani by a separation strategy involving the almost exclusive use of centrifugal partition chromatography. All five compounds were antifungal in a tie bioautography test using Cladosporium cucumerinum. Compounds 1-4 exhibited larvicidal activity against Aedes aegypti.