THE ELIMINATION OF O-LINKED GLYCANS FROM GLYCOPROTEINS UNDER NONREDUCING CONDITIONS

被引：36

作者：

COOPER, CA ^{[1
]}

PACKER, NH ^{[1
]}

REDMOND, JW ^{[1
]}

机构：

[1] MACQUARIE UNIV,SCH CHEM,N RYDE,NSW 2109,AUSTRALIA

来源：

GLYCOCONJUGATE JOURNAL | 1994年 / 11卷 / 02期

关键词：

O-LINKED SUGARS; MUCIN; GLYCOPROTEIN; BETA-ELIMINATION; SUGAR HYDRAZONES; REDUCING SUGARS;

D O I：

10.1007/BF00731156

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive beta-elimination of O-glycans.

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收藏

页码：163 / 167

页数：5

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