IMMUNOHISTOCHEMICAL LOCALIZATION OF RAF PROTEIN-KINASE IN DENDRITIC SPINES AND SPINE APPARATUSES OF THE RAT CEREBRAL-CORTEX

被引：20

作者：

MIHALY, A

ORAVECZ, T

OLAH, Z

RAPP, UR

机构：

[1] NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701

[2] MAX PLANCK INST BIOPHYS CHEM,DEPT NEUROBIOL,W-3400 GOTTINGEN,GERMANY

[3] HUNGARIAN ACAD SCI,BIOL RES CTR,INST GENET,H-6701 SZEGED,HUNGARY

[4] NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892

来源：

BRAIN RESEARCH | 1991年 / 547卷 / 02期

关键词：

PROTOONCOGENE; DENDRITIC SPINE; SPINE APPARATUS; PROTEIN KINASE; IMMUNOHISTOCHEMISTRY;

D O I：

10.1016/0006-8993(91)90976-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The ultrastructural localization of raf protein (product of the raf protooncogene) in the neocortex, pyriform cortex and hippocampus of the rat has been investigated by means of pre-embedding immunohistochemistry. Specificity of the antiserum was tested with Western blotting. Besides the immunoreactivity of the dendrites, remarkably strong immunostaining of the dendritic spines and spine apparatuses was noted in each of the investigated areas. The postsynaptic densities were also stained. Since raf proteins are serine/threonine-specific protein kinases, our findings could be important steps toward the understanding of dendritic spine plasticity.

引用

页码：309 / 314

页数：6

共 28 条

[1] CHANGES IN THE NUMERICAL DENSITY OF SYNAPTIC CONTACTS WITH LONG-TERM POTENTIATION IN THE HIPPOCAMPAL DENTATE GYRUS [J].