A MONOCLONAL-ANTIBODY AGAINST PERIPHERAL BENZODIAZEPINE RECEPTOR ACTIVATES THE HUMAN NEUTROPHIL NADPH-OXIDASE

被引：25

作者：

ZAVALA, F ^{[1
]}

MASSON, A ^{[1
]}

BRYS, L ^{[1
]}

DEBAETSELIER, P ^{[1
]}

DESCAMPSLATSCHA, B ^{[1
]}

机构：

[1] VRIJE UNIV BRUSSELS,INST MOLEC BIOL,B-1640 RHODE ST GENESE,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90468-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A murine monoclonal antibody, mAb 8523, raised against whole human pro-monocytic U937 cells recognizes an 18 kDa antigen in human neutrophils (PMN), as determined by immunoprecipitation and by immunodetection on Western blots of SDS-PAGE of PMN membrane fractions. That this 18 kDa antigen corresponds to the phagocyte peripheral benzodiazepine receptor (PBZDR) is evidenced by its co-migration with 18 kDa covalently labeled PBZDR, detected by autoradiography, and their co-modulation upon phorbol-myristate-acetate activation of PMN. Purified mAb 8523 (IgG2b) is able to dose-dependently and specifically stimulate both the basal and the FMLP-induced oxidative burst of intact human PMN, assessed by luminol-amplified chemiluminescence. This property of the first described monoclonal antibody against PBZDR supports the implication of this receptor in NADPH-oxidase activation and consequently in phagocyte-dependent host defense mechanisms. © 1991.

引用

页码：1577 / 1583

页数：7

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