INFLUENCE OF TEMPERATURE ON THE SIEVING EFFECT OF DIFFERENT POLYMER MATRICES IN CAPILLARY SDS GEL-ELECTROPHORESIS OF PROTEINS

被引：90

作者：

GUTTMAN, A

HORVATH, J

COOKE, N

机构：

[1] Beckman Instruments, Inc., Fullerton

[2] Hafslund Nycomed Pharma, Linz, Austria, A-4021

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 03期

关键词：

D O I：

10.1021/ac00051a002

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two different sieving polymers, branched (dextran) and linear (poly(ethylene oxide)), were used in high-performance capillary gel electrophoresis to study the effect of temperature on the separation of SDS-protein complexes in the molecular mass range of 14 400-97 400 Da. Migration properties and band broadening of the proteins were studied as a function of column temperature ranging from 20 and 50-degrees-C. Equations have been derived that relate the effect of temperature on the mobility of the solute. Using an isoelectrostatic separation mode, the migration time of the SDS-protein molecules decreased in both instances as temperature increased within the examined time interval. Peak efficiency increased with elevated temperature in the dextran-based column but decreased when PEO was used as a sieving material.

引用

页码：199 / 203

页数：5

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