SYNGENEIC BONE-MARROW TRANSPLANTATION ELIMINATES V-BETA-8.2 T-LYMPHOCYTES FROM THE SPINAL-CORD OF LEWIS RATS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

被引：40

作者：

BURT, RK ^{[1
]}

BURNS, W ^{[1
]}

RUVOLO, P ^{[1
]}

FISCHER, A ^{[1
]}

SHIAO, C ^{[1
]}

GUIMARAES, A ^{[1
]}

BARRETT, J ^{[1
]}

HESS, A ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV HOSP,CTR ONCOL,BONE MARROW TRANSPLANTAT UNIT,BALTIMORE,MD

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1995年 / 41卷 / 04期

关键词：

EAE; MS; MBP;

D O I：

10.1002/jnr.490410412

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS), is a paralytic disease of the central nervous system (CNS) mediated by T-lymphocytes reactive to myelin basic protein (MBP), Lewis rats actively immunized with fragment 68 to 82 of guinea pig MBP develop a monophasic disease with spontaneous recovery, Lymphocyte recognition of the primary encephalitogenic sequence of MBP (fragment 68 to 82) is V(beta)8.2 T cell receptor (TCR) skewed [1-3]. Lewis rats in clinical remission at 1 month and 3 months after spontaneous resolution of EAE retain V(beta)8.2 T-lymphocytes in the CNS when analyzed by reverse transcriptase polymerase chain reaction or in situ hybridization, In contrast, 1 and 3 months after clinical remission from syngeneic bone marrow transplantation, V(beta)8.2 T lymphocytes are absent from the CNS. During clinically active EAE and inflammatory breakdown of the blood-brain barrier, immune ablation and reconstitution with syngeneic bone marrow results in clinical tolerance of the new immune system to myelin. (C) 1995 Wiley-Liss, Inc.

引用

页码：526 / 531

页数：6