A PIVOTAL ROLE FOR THE STRUCTURE OF THE HOLLIDAY JUNCTION IN DNA BRANCH MIGRATION

被引:83
作者
PANYUTIN, IG [1 ]
BISWAS, I [1 ]
HSIEH, P [1 ]
机构
[1] NIDDKD, GENET & BIOCHEM BRANCH, BETHESDA, MD 20892 USA
关键词
BRANCH MIGRATION; HOLLIDAY JUNCTION; RECOMBINATION;
D O I
10.1002/j.1460-2075.1995.tb07170.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Branch migration of a DNA Holliday junction is a key step in genetic recombination that affects the extent of transfer of genetic information between homologous DNA sequences. We previously observed that the rate of spontaneous branch migration is exceedingly sensitive to metal ions and postulated that. the structure of the cross-over point might be one critical determinant of the rate of branch migration. Other investigators have shown that in the presence of divalent metal ions like magnesium, the Holliday junction assumes a folded conformation in which base stacking is retained through the cross-over point. This base stacking is disrupted in the absence of magnesium. Here we measure the rate of branch migration as a function of Mg2+ concentration. The rate of branch migration increases dramatically at MgCl2 concentrations below 500 mu M, with the steepest acceleration occurring between 300 and 100 mu M MgCl2. This increase in the rate of branch migration coincides with the loss of base stacking in the four-way junction over this same interval of magnesium concentration, as measured by the susceptibility of junction residues to modification by osmium tetroxide and diethyl pyrocarbonate. We conclude that at physiological concentrations of intracellular Mg2+, base stacking in the Holliday junction constitutes one kinetic barrier to branch migration and that disruption of base stacking at the cross-over relieves this constraint.
引用
收藏
页码:1819 / 1826
页数:8
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