EFFICIENT PLANT-REGENERATION FROM CELL-SUSPENSION PROTOPLASTS OF THE WOODY MEDICINAL PLANT SOLANUM-DULCAMARA L (BITTERSWEET, WOODY NIGHTSHADE)

被引：3

作者：

CHAND, PK ^{[1
]}

DAVEY, MR ^{[1
]}

POWER, JB ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT BOT,PLANT GENET MANIPULAT GRP,UNIV PK,NOTTINGHAM NG7 2RD,ENGLAND

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 22卷 / 02期

关键词：

enzyme purification; plant regeneration; protoplast culture; Solanum dulcamara L. (bittersweet woody nightshade) cell suspensions;

D O I：

10.1007/BF00043687

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Large populations of viable protoplasts were released from suspension cultured cells of the woody medicinal plant Solanum dulcamara (bittersweet, woody nightshade) when the cells were harvested 3 to 7 months after culture initiation and 4 to 5 days after transfer to fresh medium. A Bio-Gel p6 purified enzyme mixture enhanced the protoplast plating efficiency 6 fold compared to the unpurified mixture, without affecting protoplast yield. Agarose-solidified medium markedly improved protoplast division and colony formation, and enabled protoplasts to be plated at lower densities than in liquid medium. All protoplast-derived tissues produced shoots on MS based medium with 1.0 mgl-1 zeatin. Shoots rooted readily on medium lacking phytohormones. Cytological examination revealed high chromosome stability of suspension cultured cells, of plants derived from such cells, and of protoplast-derived plants. The implication of these results is discussed in relation to the genetic manipulation of this pharmaceutically important plant. © 1990 Kluwer Academic Publishers.

引用

页码：119 / 125

页数：7

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