EVALUATION OF FINGERPRINTING METHODS FOR IDENTIFICATION OF HELICOBACTER-PYLORI STRAINS

被引：15

作者：

CLAYTON, CL ^{[1
]}

KLEANTHOUS, H ^{[1
]}

DENT, JC ^{[1
]}

MCNULTY, CAM ^{[1
]}

TABAQCHALI, S ^{[1
]}

机构：

[1] GLOUCESTER ROYAL HOSP,PUBL HLTH LAB,GLOUCESTER,ENGLAND

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1991年 / 10卷 / 12期

基金：

英国惠康基金;

关键词：

D O I：

10.1007/BF01984926

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Variation amongst strains of Helicobacter pylori was examined by S-35-methionine-labelled protein SDS-PAGE (Radio-PAGE), immunoblot and DNA fingerprinting techniques. These methods allowed discrimination amongst isolates and showed total correlation. Pig and baboon gastric Helicobacter pylori-like organisms were found to be very similar to Helicobacter pylori by both Radio-PAGE and immunoblotting, whereas a Helicobacter mustelae isolate was markedly different. The HindIII restriction endonuclease digest patterns of Helicobacter pylori DNA illustrated the considerable genomic variation of this organism. The Radio-PAGE and immunoblot typing methods both gave precise identification of Helicobacter pylori strains, but Radio-PAGE was found to give higher resolution and represents a standardised universally applicable fingerprinting method for Helicobacter pylori.

引用

页码：1040 / 1047

页数：8

共 41 条

[1] ASCENCIO F, 1990, HELICOBACTER PYLORI, P104
[2] BEJI A, 1989, LANCET, V2, P1402
[3] BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[4] GASTRIC CAMPYLOBACTER-LIKE ORGANISMS, GASTRITIS, AND PEPTIC-ULCER DISEASE
BLASER, MJ
[J]. GASTROENTEROLOGY, 1987, 93 (02) : 371 - 383
[5] BLASER MJ, 1990, HELICOBACTER PYLORI, P38
[6] INVESTIGATION OF A CAMPYLOBACTER-JEJUNI OUTBREAK BY SEROTYPING AND CHROMOSOMAL RESTRICTION ENDONUCLEASE ANALYSIS
BRADBURY, WC
PEARSON, AD
MARKO, MA
CONGI, RV
PENNER, JL
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1984, 19 (03) : 342 - 346
[7] IMMUNOBLOT FINGERPRINTING OF CAMPYLOBACTER-PYLORI
BURNIE, JP
LEE, W
DENT, JC
MCNULTY, CAM
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1988, 27 (02) : 153 - 159
[8] MOLECULAR-CLONING AND EXPRESSION OF CAMPYLOBACTER-PYLORI SPECIES-SPECIFIC ANTIGENS IN ESCHERICHIA-COLI K-12
CLAYTON, CL
WREN, BW
MULLANY, P
TOPPING, A
TABAQCHALI, S
[J]. INFECTION AND IMMUNITY, 1989, 57 (02) : 623 - 629
[9] CLAYTON CL, 1990, NUCLEIC ACIDS RES, V18, P363
[10] COGHLAN JG, 1987, LANCET, V2, P1109

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