STAINING PLANT-CELLS WITH SILVER .1. THE SALT-NYLON TECHNIQUE

被引：37

作者：

STACK, S ^{[1
]}

HERICKHOFF, L ^{[1
]}

SHERMAN, J ^{[1
]}

ANDERSON, L ^{[1
]}

机构：

[1] COLORADO STATE UNIV, CELL & MOLEC BIOL PROGRAM, FT COLLINS, CO 80523 USA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1991年 / 66卷 / 02期

基金：

美国国家科学基金会;

关键词：

SILVER STAINING; PLANT CELLS; SYNAPTONEMAL COMPLEX; NUCLEOLUS; NUCLEOLUS ORGANIZER REGION; KINETOCHORE; CHROMOSOME CORE;

D O I：

10.3109/10520299109110553

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A technique is described for selectively silver staining nucleoli, active nucleolus organizers, nucleolar material attached to chromosomes, kinetochores, synaptonemal complexes, and chromosome cores in plant cells. The technique, called salt-nylon silver staining, involves spreading cells on glass slides, treating the cells with a solution of saline sodium citrate, and incubating the cells in a silver nitrate solution covered with nylon screen. Selected variables important for achieving reliable silver staining are considered.

引用

页码：69 / 78

页数：10

共 27 条

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