PHYSICAL ASSOCIATION OF RAS AND CATHEPSIN-B AND CATHEPSIN-L IN THE CONDITIONED MEDIUM OF V-HA-RAS-TRANSFORMED NIH3T3 CELLS

被引：13

作者：

HIWASA, T ^{[1
]}

KOMINAMI, E ^{[1
]}

机构：

[1] JUNTENDO UNIV,SCH MED,DEPT BIOCHEM,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 216卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2696

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoblot analysis of the conditioned medium of v-Ha-ras-transformed NIH3T3 cells showed that Ras protein is secreted in the culture fluid. Because cathepsins L and B whose activities are inhibited by Ras are also secreted, possible association between Ras and cathepsins was investigated by immunoprecipitation and following immunoblot analysis. The results showed that Ras is physically associated with cathepsins B and L in the culture fluid. (C) 1995 Academic Press, Inc.

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页码：828 / 834

页数：7

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