STRUCTURE OF THE ECDYSONE GLUCOSIDE FORMED BY A BACULOVIRUS ECDYSTEROID UDP-GLUCOSYLTRANSFERASE

被引：31

作者：

OREILLY, DR

HOWARTH, OW

REES, HH

MILLER, LK

机构：

[1] UNIV GEORGIA,DEPT GENET,ATHENS,GA 30602

[2] UNIV WARWICK,DEPT CHEM,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

[3] UNIV LIVERPOOL,DEPT BIOCHEM,LIVERPOOL L69 3BX,ENGLAND

来源：

INSECT BIOCHEMISTRY | 1991年 / 21卷 / 07期

关键词：

AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS; BACULOVIRUS; ECDYSTEROID UDP-GLUCOSYLTRANSFERASE; ECDYSONE GLUCOSIDE;

D O I：

10.1016/0020-1790(91)90121-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The egt gene of the baculovirus Autographa californica nuclear polyhedrosis virus encodes an ecdysteroid UDP-glucosyltransferase. The glucoside formed by this enzyme using ecdysone and UDP-glucose as substrates has been purified and structurally characterized by nuclear magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry. These studies have identified the conjugate as ecdysone 22-O-beta-D-glucopyranoside. Substrate specificity studies have confirmed that ecdysteroids lacking a hydroxyl moiety at C-22 are not substrates for the enzyme.

引用

页码：795 / 801

页数：7

共 15 条

[1] INVESTIGATION OF COMPLEX NETWORKS OF SPIN-SPIN COUPLING BY TWO-DIMENSIONAL NMR [J].