A DELAYED AND SUSTAINED RISE OF CYTOSOLIC CALCIUM IS ELICITED BY OXIDIZED LDL IN CULTURED BOVINE AORTIC ENDOTHELIAL-CELLS

被引：70

作者：

NEGRESALVAYRE, A ^{[1
]}

FITOUSSI, G ^{[1
]}

REAUD, V ^{[1
]}

PIERAGGI, MT ^{[1
]}

THIERS, JC ^{[1
]}

SALVAYRE, R ^{[1
]}

机构：

[1] UNIV TOULOUSE 3,FAC MED RANGUEIL,DEPT BIOCHEM,METAB DIS LAB,F-31062 TOULOUSE,FRANCE

来源：

FEBS LETTERS | 1992年 / 299卷 / 01期

关键词：

CALCIUM; CYTOTOXICITY; OXIDIZED LDL; ENDOTHELIAL CELL; ATHEROSCLEROSIS; QUIN-2/AM; FLUO-3/AM;

D O I：

10.1016/0014-5793(92)80101-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine aortic endothelial cells (BAEC) pulsed for 5 h with mildly oxidized low density lipoproteins (LDL), exhibited a broad, sustained and high peak of [Ca2+]i occurring several hours after the end of the pulse and reaching very high [Ca2+]i values (around 2500-3000 nmol/l) and a concomitant drop of cytosolic pH (around 0.2-0.3 pH units) without any loss of cell viability. When BAEC were continuously pulsed with oxidized LDL, the peak of [Ca2+]i was more sustained than in short pulse experiments and was associated with irreversible morphological changes usually associated with cytotoxic events (blebbing) and with a marked loss of viability. The potential involvement of these biochemical and morphological changes in atherogenesis are discussed.

引用

页码：60 / 65

页数：6

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