DETECTION OF PEA SEED-BORNE MOSAIC POTYVIRUS BY SEQUENCE SPECIFIC ENZYMATIC AMPLIFICATION

被引：21

作者：

KOHNEN, PD

DOUGHERTY, WG

HAMPTON, RO

机构：

[1] OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,USDA ARS,CORVALLIS,OR 97331

[2] OREGON STATE UNIV,DEPT MICROBIOL,CORVALLIS,OR 97331

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 37卷 / 03期

关键词：

PSBMV; PCR; SEED-TRANSMISSION; VIRUS DETECTION;

D O I：

10.1016/0166-0934(92)90027-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction (PCR) was used to detect pea seedborne mosaic potyvirus (PSbMV) pathotype P1 RNA after reverse transcription of total nucleic acid preparations from pea (Pisum sativum) tissues. Tissues assayed for PSbMV included leaves, roots, petals, seed parts, and pollen. Three oligonucleotide primers in appropriate combination yielded two products of the predicted size: 730 and 1200 bp. The described methodology allows for rapid pathotype-specific PSbMV detection with utmost sensitivity and wide applicability.

引用

页码：253 / 258

页数：6

共 6 条

[1] 3 PEA SEED-BORNE MOSAIC-VIRUS PATHOTYPES FROM PEA AND LENTIL GERM PLASM [J].