DIRECT IMAGING IN A WATER LAYER OF HUMAN-CHROMOSOME FIBERS COMPOSED OF NUCLEOSOMES AND THEIR HIGHER-ORDER STRUCTURES BY LASER-PLASMA X-RAY CONTACT MICROSCOPY

被引：20

作者：

KINJO, Y

SHINOHARA, K

ITO, A

NAKANO, H

WATANABE, M

HORIIKE, Y

KIKUCHI, Y

RICHARDSON, MC

TANAKA, KA

机构：

[1] TOKYO METROPOLITAN INST MED SCI,DEPT RADIAT RES,TOKYO 113,JAPAN

[2] HIROSHIMA UNIV,DEPT ENGN,HIROSHIMA 724,JAPAN

[3] TOSHIBA CO LTD,ULSI RES CTR,KAWASAKI,KANAGAWA 210,JAPAN

[4] UNIV CENT FLORIDA,CTR RES ELECTROOPT & LASERS,ORLANDO,FL 32826

[5] OSAKA UNIV,DEPT ELECTROMAGNET ENERGY ENGN,SUITA,OSAKA 565,JAPAN

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1994年 / 176卷

关键词：

LASER-PLASMA X-RAYS; CONTACT MICROSCOPY; HYDRATED HUMAN CHROMOSOME FIBER; UNFIXED; UNSTAINED; NUCLEOSOME; SUPERBEAD;

D O I：

10.1111/j.1365-2818.1994.tb03500.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

X-ray contact microscopy with a 300-ps-duration laser-plasma X-ray source has been used to image hydrated human chromosomes. Clearly imaged are individual nucleosomes and their higher-order particles (superbeads), elementary chromatin fibrils c. 30nm in diameter and their higher-order fibres of various sizes up to c. 120nm in diameter. The results demonstrate that X-ray microscopy is now capable of opening a new path of investigation into the detailed structures of hydrated chromosome fibres in their natural state.

引用

页码：63 / 74

页数：12

共 14 条

[1] SUPRANUCLEOSOMAL ORGANIZATION OF CHROMATIN - ELECTRON-MICROSCOPIC VISUALIZATION OF LONG POLYNUCLEOSOMAL CHAINS [J].