THE DETECTION OF SALMONELLA IN SKIMMED-MILK POWDER ENRICHMENTS USING CONVENTIONAL METHODS AND IMMUNOMAGNETIC SEPARATION

被引：25

作者：

DZIADKOWIEC, D ^{[1
]}

MANSFIELD, LP ^{[1
]}

FORSYTHE, SJ ^{[1
]}

机构：

[1] NOTTINGHAM TRENT UNIV,DEPT LIFE SCI,NOTTINGHAM NG11 8NS,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1995年 / 20卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1995.tb01321.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Skimmed milk powders were spiked with one of three Salmonella serovars and incubated in buffered peptone water for 24 h. No false-negative results were obtained by immunomagnetic separation (IMS), compared to seven for selenite cysteine, one for Muller-Kauffmann tetrathionate and two for Rappaport-Vassiliadis enrichment broths. Salmonella virchow was detected and enumerated during the pre-enrichment incubation by IMS and indirect conductance techniques. The Saint. virchow cell number did not increase after 12 h incubation and remained at 3 x 10(6) cfu ml(-1). IMS was able to capture Salm. virchow cells at cell numbers ca 50 ml(-1) in the presence of a 1000 greater number of non-salmonella cells.

引用

页码：361 / 364

页数：4

共 8 条

[1]

Salmonella virchow phage type 26, Communicable Disease Report, 4, (1994)

[2]

Salmonella infections, England and Wales: reports to PHLS (salmonella data set), Communicable Disease Report, 4, (1994)

[3]

Blackburn, Rapid and alternative methods for the detection of salmonellas in foods, Journal of Applied Bacteriology, 75, pp. 199-214, (1993)

[4]

Cudjoe K.S., Krona R., Olsen E., IMS: a new selective enrichment technique for detection of Salmonella in foods, International Journal of Food Microbiology, 23, pp. 159-165, (1994)

[5]

Donaghy J.A., Madden R.H., Detection of Salmonella in animal protein by Rappaport‐Vassiliadis broth using indirect impediometry, International Journal of Food Microbiology, 17, pp. 281-288, (1993)

[6]

Mansfield L.P., Forsythe S.J., Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection, Letters in Applied Microbiology, 16, pp. 122-125, (1993)

[7]

Parmar N., Easter M.C., Forsythe S.J., The detection of Salmonella enteritidis and S. typhimurium using immunomagnetic separation and conductance microbiology, Letters in Applied Microbiology, 15, pp. 175-178, (1992)

[8]

Safarik I., Forsythe S., Safarikova M., A review: The application of magnetic separations in applied microbiology, Journal of Applied Bacteriology, 78, (1995)

← 1 →