COEXPRESSION OF SLOW-TWITCH/CARDIAC MUSCLE CA2+-ATPASE (SERCA2) AND PHOSPHOLAMBAN

被引：41

作者：

FUJII, J

MARUYAMA, K

TADA, M

MACLENNAN, DH

机构：

[1] UNIV TORONTO,CHARLES H BEST INST,BANTING & BEST DEPT MED RES,112 COLL ST,TORONTO M5G 1L6,ONTARIO,CANADA

[2] OSAKA UNIV,SCH MED,DEPT MED & PATHOPHYSIOL 1,OSAKA 553,JAPAN

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

Ca[!sup]2+[!/sup]-affinity; Ca[!sup]2+[!/sup]-ATPase; Co-expression; Phospholamban;

D O I：

10.1016/0014-5793(90)81092-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Full length cDNAs encoding both slow-twitch/cardiac (SERCA2) and fast-twitch skeletal muscle (SERCA1) Ca2+-ATPases were expressed by transient transfection of COS-1 cells. Studies of the Ca2+-dependency of Ca2+-transport in microsomes isolated from these cells showed that both isoforms had an affinity for Ca2+ of about 0.2 μM. The Ca2+-affinity of SERCA2 was lowered when phospholamban was co-expressed with it, demonstrating that the two proteins interact in this expression system. These studies support the view that phospholamban inhibition accounts for the low Ca2+-amnity and low activity of SERCA2 in cardiac muscle sarcoplasmic reticulum. © 1990.

引用

页码：232 / 234

页数：3

共 23 条

[1] 2 CA-2+ ATPASE GENES - HOMOLOGIES AND MECHANISTIC IMPLICATIONS OF DEDUCED AMINO-ACID-SEQUENCES [J].