SECRETION OF PROSTAGLANDINS ELICITED BY LIPOPOLYSACCHARIDE AND ETHANOL IN CULTURED RAT KUPFFER CELLS

被引：24

作者：

VICTOROV, AV ^{[1
]}

HOEK, JB ^{[1
]}

机构：

[1] MINIST PUBL HLTH RUSSIA,CENT RES INST EPIDEMIOL,MOLEC MECHANISMS INFECT LAB,MOSCOW,RUSSIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2519

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prostaglandins (PGs) released by cultured rat Kupffer cells in response to stimulation with lipopolysaccharide (LPS) or ethanol were extracted from culture media, separated by HPLC and measured by radioimmunoassay. LPS (0.5-5 mu g/ml) enhanced. after a 3-4 hrs lag period. the production of PGE2 (7-10 fold by 24 hrs), thromboxane B2 (2-3 fold) and PGD2, PG 6-keto-F1 alpha. PGF2 alpha(20-50% each): This effect was not inhibited by 30 mu M aspirin but was reduced by dexamethasone. Ethanol (25-85 mM) gradually increased the release of PGE2 (40-90% by 24 hrs) and other PGs (10-30%), with 30 mu M aspirin eliminating this effect. When added together with LPS, ethanol potentiated the endotoxin action. We suggest that LPS causes synthesis of the inducible cyclooxygenase-2 form in Kupffer cells, whereas ethanol exerts its effect via the preexisting cyclooxygenase-1 mainly by increasing the free arachidonic acid content. (C) 1995 Academic Press, Inc.

引用

页码：691 / 697

页数：7

共 25 条

[1] ETHANOL INHIBITS ZYMOSAN-STIMULATED EICOSANOID PRODUCTION IN MOUSE PERITONEAL-MACROPHAGES [J].

BALSINDE, J ;

FERNANDEZ, B ;

SOLISHERRUZO, JA .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1210 (02) :195-201

[2] HEPATOCYTE MODULATION OF KUPFFER CELL PROSTAGLANDIN-E2 PRODUCTION INVITRO [J].

BILLIAR, TR ;

LYSZ, TW ;

CURRAN, RD ;

BENTZ, BG ;

MACHIEDO, GW ;

SIMMONS, RL .

JOURNAL OF LEUKOCYTE BIOLOGY, 1990, 47 (04) :304-311

[3] BIOLOGICALLY-ACTIVE PRODUCTS OF STIMULATED LIVER MACROPHAGES (KUPFFER CELLS) [J].

DECKER, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 192 (02) :245-261

[4] ETHANOL INDUCES RELEASE OF ARACHIDONIC-ACID BUT NOT SYNTHESIS OF EICOSANOIDS IN MOUSE PERITONEAL-MACROPHAGES [J].

DIEZ, E ;

BALSINDE, J ;

ARACIL, M ;

SCHULLER, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 921 (01) :82-89

[5] KUPFFER CELL-ACTIVITY AND HEPATIC MICROVASCULAR EVENTS AFTER ACUTE ETHANOL INGESTION IN MICE [J].

EGUCHI, H ;

MCCUSKEY, PA ;

MCCUSKEY, RS .

HEPATOLOGY, 1991, 13 (04) :751-757

[6]

FLISIAK R, 1993, HEPATOLOGY, V18, P153

[7]

FU JY, 1990, J BIOL CHEM, V265, P16737

[8] REGULATION OF INTERLEUKIN-6 RECEPTOR EXPRESSION IN RAT KUPFFER CELLS - MODULATION BY CYTOKINES, DEXAMETHASONE AND PROSTAGLANDIN E(2) [J].

HOFFMANN, R ;

HENNINGER, HP ;

SCHULZESPECKING, A ;

DECKER, K .

JOURNAL OF HEPATOLOGY, 1994, 21 (04) :543-550

[9] CONTRIBUTION OF PHOSPHOLIPASES A(2) AND D TO ARACHIDONIC-ACID LIBERATION AND PROSTAGLANDIN D-2 FORMATION WITH INCREASE IN INTRACELLULAR CA2+ CONCENTRATION IN RAT PERITONEAL MAST-CELLS [J].

ISHIMOTO, T ;

AKIBA, S ;

SATO, T ;

FUJII, T .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (1-2) :401-406

[10] POSSIBLE INDUCTION OF FATTY-ACID CYCLOOXYGENASE IN LIPOPOLYSACCHARIDE-STIMULATED RAT KUPFFER CELLS [J].

KAWADA, N ;

MIZOGUCHI, Y ;

KOBAYASHI, K ;

MONNA, T ;

MORISAWA, S ;

UEDA, N ;

OMOTO, Y ;

TAKAHASHI, Y ;

YAMAMOTO, S .

GASTROENTEROLOGY, 1992, 103 (03) :1026-1033

← 1 2 3 →