PRODUCTION AND SECRETION OF PLASMINOGEN IN CULTURED RAT-BRAIN MICROGLIA

被引：92

作者：

NAKAJIMA, K ^{[1
]}

TSUZAKI, N ^{[1
]}

NAGATA, K ^{[1
]}

TAKEMOTO, N ^{[1
]}

KOHSAKA, S ^{[1
]}

机构：

[1] NATL INST NEUROSCI,DEPT NEUROCHEM,4-1-1 OGAWA HIGASHI,KODAIRA,TOKYO 187,JAPAN

来源：

FEBS LETTERS | 1992年 / 308卷 / 02期

关键词：

PROTEASE; PLASMINOGEN; PLASMINOGEN ACTIVATOR; CULTURE; MICROGLIA;

D O I：

10.1016/0014-5793(92)81270-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The production and secretion of plasminogen in cultured rat brain microglia was investigated. Urokinase-dependent cascinolytic activity was detected by zymography in microglial conditioned medium with a molecular weight of about 90 kDa. The 90-kDa protein was also detected by Western blotting with anti-rat plasminogen antiserum in the non-reducing condition. Immunoprecipitation with plasminogen antiserum following [S-35]methionine labelling revealed that the plasminogen detected in microglial conditioned medium is synthesized in microglia. The amount of plasminogen in the conditioned medium was increased by stimulation with lipopolysaccharide. These results show that cultured microglia produce plasminogen and secrete it into the culture medium.

引用

页码：179 / 182

页数：4

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