ANALYSIS OF SCHIZOSACCHAROMYCES-POMBE MITOCHONDRIAL-DNA REPLICATION BY 2-DIMENSIONAL GEL-ELECTROPHORESIS

被引：37

作者：

HAN, ZY ^{[1
]}

STACHOW, C ^{[1
]}

机构：

[1] BOSTON COLL, DEPT BIOL, CHESTNUT HILL, MA 02167 USA

来源：

CHROMOSOMA | 1994年 / 103卷 / 03期

关键词：

D O I：

10.1007/BF00368008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The entire mitochondrial genome of Schizosaccharomyces pombe ura4-294h(-) was analyzed by the 2D pulsed field gel electrophoresis technique developed by Brewer and Fangman. The genome consists of multimers with an average size of 100 kb and analysis of the overlapping restriction fragments of the complete mitochondrial DNA (mtDNA) genome resulted in simple Y 2D gel patterns. Large single-stranded DNA molecules or double-stranded DNA molecules containing large or numerous single-stranded regions were found in the S. pombe mtDNA preparation. The replication of mtDNA monomers was found to occur in either direction. On the basis of these results, a replication mechanism for S. pombe mtDNA that is most consistent with a rolling circle model is suggested.

引用

页码：162 / 170

页数：9

共 38 条

[1] THE MITOCHONDRIAL GENOME OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE .1. ISOLATION AND PHYSICAL MAPPING OF MITOCHONDRIAL-DNA [J].