CLONING AND NUCLEOTIDE-SEQUENCE ANALYSIS OF THE HUMAN BETA-MICROSEMINOPROTEIN GENE

被引：27

作者：

GREEN, CB ^{[1
]}

LIU, WY ^{[1
]}

KWOK, SCM ^{[1
]}

机构：

[1] CHILDRENS HOSP LOS ANGELES, MED GENET SECT, LOS ANGELES, CA 90027 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90648-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

β-microseminoprotein (MSP) is a small protein (94 amino acids) synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. Restriction endonuclease mapping of human genomic DNA with a human MSP cDNA probe identified a 19 kilobase (Kb) hybridizing band in both EcoRI and BamHI digestions. Subsequently, the 19 Kb EcoRI fragment of human genomic DNA containing the MSP gene was isolated and cloned into an EMBL4 phage vector. Screening of the recombinant phages resulted in the isolation of one clone containing the MSP gene. Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb. © 1990.

引用

页码：1184 / 1190

页数：7

共 20 条

[1] THE AMINO-ACID SEQUENCE OF HUMAN BETA-MICROSEMINOPROTEIN [J].

AKIYAMA, K ;

YOSHIOKA, Y ;

SCHMID, K ;

OFFNER, GD ;

TROXLER, RF ;

TSUDA, R ;

HARA, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 829 (02) :288-294

[2] SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].

BENTON, WD ;

DAVIS, RW .

SCIENCE, 1977, 196 (4286) :180-182

[3] CHARON PHAGES FOR DNA CLONING .8. CLONING HUMAN FETAL GAMMA-GLOBIN AND MOUSE ALPHA-TYPE GLOBIN DNA - PREPARATION AND SCREENING OF SHOTGUN COLLECTIONS [J].

BLATTNER, FR ;

BLECHL, AE ;

DENNISTONTHOMPSON, K ;

FABER, HE ;

RICHARDS, JE ;

SLIGHTOM, JL ;

TUCKER, PW ;

SMITHIES, O .

SCIENCE, 1978, 202 (4374) :1279-1284

[4]

BRAR A, 1988, J ANDROL, V9, P253

[5]

DELSAL G, 1989, BIOTECHNIQUES, V7, P514

[6] IMMUNOHISTOCHEMICAL LOCALIZATION OF A PROSTATIC SECRETORY PROTEIN OF 94 AMINO-ACIDS IN NORMAL PROSTATIC TISSUE, IN PRIMARY PROSTATIC TUMORS AND IN THEIR METASTASES [J].

DUBE, JY ;

PELLETIER, G ;

GAGNON, P ;

TREMBLAY, RR .

JOURNAL OF UROLOGY, 1987, 138 (04) :883-887

[7]

HARA M, 1989, J LAB CLIN MED, V113, P541

[8] ANALYSIS OF AN INHIBIN PREPARATION REVEALS APPARENT IDENTITY BETWEEN A PEPTIDE WITH INHIBIN-LIKE ACTIVITY AND A SPERM-COATING ANTIGEN [J].

JOHANSSON, J ;

SHETH, A ;

CEDERLUND, E ;

JORNVALL, H .

FEBS LETTERS, 1984, 176 (01) :21-26

[9]

KRUG MS, 1987, METHOD ENZYMOL, V152, P316

[10]

KWOK SCM, 1981, BIOCHEM BIOPH RES CO, V98, P844

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