CRYOPRESERVED CYTOPLASTS FROM HUMAN POLYMORPHONUCLEAR LEUKOCYTES (CYTOKINEPLASTS) ARE CHEMOTACTIC AT SPEEDS COMPARABLE TO THOSE OF FRESH INTACT-CELLS

被引：13

作者：

MALAWISTA, SE ^{[1
]}

DEBOISFLEURYCHEVANCE, A ^{[1
]}

机构：

[1] HOP LA PITIE SALPETRIERE,CTR ETUDES ECOL CELLULES SANG NORMALES & LEUCEM,DEPT HEMATOL,F-75651 PARIS 13,FRANCE

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1991年 / 50卷 / 03期

关键词：

LOCOMOTION; PMN; MOTILITY;

D O I：

10.1002/jlb.50.3.313

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cytokineplasts (CKP) are granule-poor cytoplasts from human polymorphonuclear leukocytes (PMN) that retain motile function, even (unlike the parent PMN) after cryopreservation. Employing time-lapse videomicroscopy, we examined the chemotactic properties of CKP after cryopreservation toward erythrocytes lysed by laser microirradiation. Paths of locomotion were plotted for six CKP in the field, and velocities were calculated at 10-sec intervals. Mean velocities of the six fragments, ranging from 9.3 to 20.8-mu-m/min, are of the order of fresh, intact PMN, the fastest of locomoting cells.

引用

页码：313 / 315

页数：3

共 12 条

[1]

BESSIS M, 1973, NOUV REV FR HEMATOL, V13, P285

[2] CHEMOATTRACTANT-STIMULATED POLYMORPHONUCLEAR LEUKOCYTES CONTAIN 2 POPULATIONS OF ACTIN-FILAMENTS THAT DIFFER IN THEIR SPATIAL DISTRIBUTIONS AND RELATIVE STABILITIES [J].