APPLICATION OF A PELLICULAR ANION-EXCHANGE RESIN TO THE SEPARATION OF INORGANIC AND ORGANIC-ANIONS BY SINGLE-COLUMN ANION-EXCHANGE

Applications are shown of a pellicular anion-exchange resin, prepared by hydrophobically coating a quaternized latex on a mono-disperse, non-porous resin. The low capacity material is ideal for single-column ion chromatography with both conductivity and indirect ultraviolet detection. The pellicular nature of the resin allows rapid isocratic and gradient separations. The resin shows excellent efficiency and is stable under high pressures and over a wide pH range. Stability of the coated resin to pressure, solvents and buffers is discussed. The separation of common inorganic anions, including fluoride, phosphate, chloride, nitrite, bromide, nitrate and sulfate, can be performed at levels below 1 ppm by direct injection. A large number of eluent ions and pH values can be used to optimize the selectivity. Organic acids can be separated rapidly. A rapid separation (11 min) of the common 5'-nucleotide phosphates was performed on the 15-cm column. In addition, a high-efficiency separation of the nucleotides and co-enzymes was performed on a 25-cm column. The elution order of bases was different when performing the separation with acidic and basic eluents.